Ultrasonication-induced Amyloid Fibril Formation of β2-Microglobulin
نویسندگان
چکیده
منابع مشابه
The role of conformational flexibility in β2-microglobulin amyloid fibril formation at neutral pH
RATIONALE Amyloid formation is implicated in a number of human diseases. β(2)-Microglobulin (β(2)m) is the precursor protein in dialysis-related amyloidosis and it has been shown that partial, or more complete, unfolding is key to amyloid fibril formation in this pathology. Here the relationship between conformational flexibility and β(2)m amyloid formation at physiological pH has been investig...
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Although the molecular mechanisms underlying the pathology of amyloidoses are not well understood, the interaction between amyloid proteins and cell membranes is thought to play a role in several amyloid diseases. Amyloid fibrils of β2-microglobulin (β2m), associated with dialysis-related amyloidosis (DRA), have been shown to cause disruption of anionic lipid bilayers in vitro. However, the eff...
متن کاملIntermolecular Alignment in β2-Microglobulin Amyloid Fibrils
The deposition of amyloid-like fibrils, composed primarily of the 99-residue protein β2-microglobulin (β2m), is one of the characteristic symptoms of dialysis-related amyloidosis. Fibrils formed in vitro at low pH and low salt concentration share many properties with the disease related fibrils and have been extensively studied by a number of biochemical and biophysical methods. These fibrils c...
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Bakthisaran RAMAN*†, Tadato BAN†, Miyo SAKAI†, Saloni Y. PASTA*, Tangirala RAMAKRISHNA*, Hironobu NAIKI‡, Yuji GOTO†1 and Ch. Mohan RAO*1 *Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India, †Institute for Protein Research, Osaka University, and CREST, Japan Science and Technology Agency, Yamadaoka 3-2, Suita, Osaka 565–0871, Japan, and ‡Faculty of Medical Science, ...
متن کاملRole of the C-terminal 28 residues of beta2-microglobulin in amyloid fibril formation.
Beta2microglobulin (beta2m) is the major protein component of the fibrillar amyloid deposits isolated from patients diagnosed with dialysis-related amyloidosis (DRA). While investigating the molecular mechanism of amyloid fibril formation by beta2m, we found that the beta2m C-terminal peptide of 28 residues (cbeta2m) itself forms amyloid fibrils. When viewed by electron microscopy, cbeta2m aggr...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m506501200